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reproductive tract  (Spatial Transcriptomics Inc)

 
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    Spatial Transcriptomics Inc reproductive tract
    Reproductive Tract, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reproductive tract/product/Spatial Transcriptomics Inc
    Average 86 stars, based on 1 article reviews
    reproductive tract - by Bioz Stars, 2026-06
    86/100 stars

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    Study design . Workflow for characterizing the microbiome (16S rRNA sequencing; DNA-based) and microbiota (meta-transcriptomics; RNA-based) in vaginal swabs, endometrial brushes, and endometrial biopsies. Taxonomy was assigned via the Kraken2/Bracken methodology, and in silico decontamination was performed via the Decontam R package. Characterization included abundance, diversity, and correlation between techniques. The study cohort consisted of 44 reproductive-aged women. In the validation cohort (N = 5), a dual approach (DNA- and RNA-based) was applied to all three sample types. Additional validation analyses for endometrial biopsies were performed via real-time quantitative PCR (qPCR) and microbial culture.

    Journal: Human Reproduction Open

    Article Title: Comprehensive 16S rRNA gene sequencing and meta-transcriptomic analyses of the female reproductive tract microbiota: two molecular profiles with different messages

    doi: 10.1093/hropen/hoag001

    Figure Lengend Snippet: Study design . Workflow for characterizing the microbiome (16S rRNA sequencing; DNA-based) and microbiota (meta-transcriptomics; RNA-based) in vaginal swabs, endometrial brushes, and endometrial biopsies. Taxonomy was assigned via the Kraken2/Bracken methodology, and in silico decontamination was performed via the Decontam R package. Characterization included abundance, diversity, and correlation between techniques. The study cohort consisted of 44 reproductive-aged women. In the validation cohort (N = 5), a dual approach (DNA- and RNA-based) was applied to all three sample types. Additional validation analyses for endometrial biopsies were performed via real-time quantitative PCR (qPCR) and microbial culture.

    Article Snippet: To ensure minimal contamination with bacteria from the lower reproductive tract, an endometrial brush was used with a Tao Brush IUMC (Cook Medical, Madrid, Spain), which was carefully closed within the uterine cavity after sample collection.

    Techniques: Sequencing, In Silico, Biomarker Discovery, Real-time Polymerase Chain Reaction

    Microbial composition along the female reproductive tract revealed by DNA- and RNA-based techniques . Relative abundance of bacterial genera in vaginal ( A ), endometrial brush ( B ), and endometrial ( C ) biopsies detected via 16S rRNA gene sequencing (DNA-based approach). ( D ) Microbial taxa in endometrial biopsies assigned via a meta-transcriptomic (RNA-based) approach. The microbial composition is plotted after decontamination. The samples on the iris plots are arranged by their rotational position around the origin of a principal component analysis (PCA) plot. Centred log ratio (CLR) transformation was applied to normalise the microbial counts at the genus level. Genera with abundances less than 1% were grouped as ‘Others’.

    Journal: Human Reproduction Open

    Article Title: Comprehensive 16S rRNA gene sequencing and meta-transcriptomic analyses of the female reproductive tract microbiota: two molecular profiles with different messages

    doi: 10.1093/hropen/hoag001

    Figure Lengend Snippet: Microbial composition along the female reproductive tract revealed by DNA- and RNA-based techniques . Relative abundance of bacterial genera in vaginal ( A ), endometrial brush ( B ), and endometrial ( C ) biopsies detected via 16S rRNA gene sequencing (DNA-based approach). ( D ) Microbial taxa in endometrial biopsies assigned via a meta-transcriptomic (RNA-based) approach. The microbial composition is plotted after decontamination. The samples on the iris plots are arranged by their rotational position around the origin of a principal component analysis (PCA) plot. Centred log ratio (CLR) transformation was applied to normalise the microbial counts at the genus level. Genera with abundances less than 1% were grouped as ‘Others’.

    Article Snippet: To ensure minimal contamination with bacteria from the lower reproductive tract, an endometrial brush was used with a Tao Brush IUMC (Cook Medical, Madrid, Spain), which was carefully closed within the uterine cavity after sample collection.

    Techniques: Sequencing, Transformation Assay